MTHFS

Alternative Names: 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase), Methenyl-THF synthetase, FLJ30410, HsT19268, EC 6.3.3.2.

MTHFS is a cytosolic protein involved in formate metabolism. It utilizes a magnesium cofactor to catalyze an ATP-dependent reaction that converts 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate (MTHF). MTHF is then used as a substrate by MTHFR to produce 5-MTHF, and also serves as a coenzyme in thymidine biosynthesis by thymidylate synthase (FAD).

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  • Sku: PSB-enz-096-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human 5,10-Methenyltetrahydrofolate Synthetase is an enzyme belonging to the synthetase family that plays a key role in the folate metabolic pathway. This enzyme is responsible for the synthesis of 5,10-methenyltetrahydrofolate, a crucial intermediate in the production of nucleotides and amino acids. With a molecular weight of approximately 45 kDa, Recombinant Human 5,10-Methenyltetrahydrofolate Synthetase is essential for maintaining proper cellular function and metabolism.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The MTHFS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 5mM DTT and 30% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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MTHFS
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