mPRMT1

Alternative Names: ANM1, HCP1, HRMT1L2, IR1B4, EC 2.1.1, Interferon receptor 1-bound protein 4, Protein arginine N-methyltransferase 1, Prmt1, AW214366, 6720434D09Rik.

PRMT1 is an enzyme that adds methylation marks to arginyl residues in a specific domain rich in glycine and arginine. It can also methylate other proteins such as HNRNPA1 and histones, as well as SUPT5H. Specifically, PRMT1 acts as a methyltransferase for histone H4. It is an important factor in the development of cancer and could be a potential target for novel therapies. PRMT1-mediated methylation positively affects the IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. Furthermore, the protein CAF1 has been identified as a regulator of PRMT1-dependent arginine methylation. PRMT1 also plays a role in regulating the activity of the MRE11-RAD50-NBS1 complex, which is involved in DNA damage checkpoint response during the intra-S-phase of the cell cycle. Additionally, PRMT1 is involved in regulating transcriptional activity. Although PRMT1 is primarily located in the cytoplasm, a fraction of it can be found in the nucleus.

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  • Sku: PSB-enz-365-10µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Mouse Protein Arginine Methyltransferase 1 (PRMT1) is an enzyme that plays a critical role in the post-translational modification of proteins by catalyzing the transfer of methyl groups from S-adenosyl-L-methionine to arginine residues. Belonging to the PRMT family, PRMT1 is involved in various cellular processes, including gene expression regulation, RNA processing, and signal transduction. With a molecular weight of approximately 45 kDa, this enzyme is crucial for maintaining proper cellular function and is a potential target for therapeutic interventions in diseases associated with dysregulated protein methylation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Specific activity is defined as the amount of enzyme that transfer 1.0nmole of methyl group per minute at 37C and was found to be > 30nmol/min/mg.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The PRMT1 solution (1mg/ml) contains 40mM Tris-HCl pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT and 40% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGILCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYVHALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mPRMT1
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