MPI

Alternative Names: Phosphomannose isomerase, Mannose-6-phosphate isomerase, Phosphohexomutase, PMI1, CDG1B, EC 5.3.1.8, FLJ39201.

MPI is a member of the mannose-6-phosphate isomerase type 1 family, abundantly expressed in the heart, brain, and skeletal muscle. This enzyme is localized to the cytoplasm and uses zinc as a cofactor to facilitate the interconversion of fructose-6-phosphate and mannose-6-phosphate. Mutations in the MPI gene are responsible for causing carbohydrate-deficient glycoprotein syndrome, type Ib.

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  • Sku: PSB-enz-169-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Mannose Phosphate Isomerase is a protein belonging to the isomerase enzyme family. With a molecular weight of approximately 64 kDa, this enzyme plays a key role in the conversion of mannose-6-phosphate to fructose-6-phosphate in the glycolytic pathway. Its recombinant form is commonly used in research and biotechnological applications for its ability to catalyze this specific biochemical reaction with high efficiency and specificity.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The MPI solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 5% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKTAAGNNME DIFGELLLQL HQQYPGDIGC FAIYFLNLLT LKPGEAMFLE ANVPHAYLKG DCVECMACSD NTVRAGLTPK FIDVPTLCEM LSYTPSSSKD RLFLPTRSQE DPYLSIYDPP VPDFTIMKTE VPGSVTEYKV LALDSASILL MVQGTVIAST PTTQTPIPLQ RGGVLFIGAN ESVSLKLTEP KDLLIFRACC LL

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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MPI

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