MPG

Alternative Names: 3-methyladenine DNA glycosidase, 3-alkyladenine DNA glycosylase, N-methylpurine-DNA glycosylase, DNA-3-methyladenine glycosylase, ADPG, MPG, AAG, ANPG, MID1, MDG, PIG11, PIG16, CRA36.1.

DNA-3-methyladenine glycosylase (MPG) is a type of enzyme that belongs to the DNA glycosylase MPG family. MPG plays a role in base excision repair by eliminating different types of purine adducts caused by alkylation, deamination, and lipid peroxidation in DNA.

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  • Sku: PSB-enz-151-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human N-Methylpurine-DNA Glycosylase is an enzyme that belongs to the DNA glycosylase/AP lyase family. This enzyme plays a crucial role in the repair of DNA damage caused by alkylating agents. With a molecular weight of approximately 23 kDa, Recombinant Human N-Methylpurine-DNA Glycosylase is involved in the removal of N-methylpurine adducts from DNA, thereby preventing mutations and maintaining genomic stability. This enzyme is commonly used in research and biotechnology applications for its ability to specifically recognize and excise damaged DNA bases.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
33.9 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation MPG protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol, 200mM NaCl and 1mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQALE HHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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MPG

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