MMP3 HEK

Alternative Names: Matrix metalloproteinase-3, MMP-3, Stromelysin-1, EC 3.4.24.17, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.

MMP-3, also known as Stromelysin-1 or Transin-1, is an enzyme that breaks down natural collagen at physiological pH and temperature. It is involved in degrading the intervertebral nucleus pulposus and annulus fibrosus of herniated lumbar intervertebral discs. MMP-3 targets various components of the extracellular matrix such as proteoglycan, laminin, fibronectin, gelatin, and collagen types III, IV, and IX. Additionally, MMP-3 can activate other matrix metalloproteinases like pro-MMP-9 and pro-MMP-8, and enhance the activity of plasmin-activated MMP-1. MMP-3 is initially secreted as an inactive form and becomes active through the action of various proteinases such as plasmin, trypsin, chymotrypsin, cathepsin G, or human neutrophil elastase. It has also been shown to activate the precursor of interleukin-1 beta.

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  • Sku: PSB-enz-284-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Matrix Metalloproteinase-3, HEK is a member of the matrix metalloproteinase family, specifically belonging to the subgroup of enzymes that degrade the extracellular matrix. This protein plays a role in tissue remodeling and is involved in various physiological processes. With a molecular weight of approximately 57 kDa, Recombinant Human Matrix Metalloproteinase-3, HEK is commonly used in research and drug development studies due to its ability to cleave a wide range of substrates.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System HEK 293
Purity 95% by SDS-PAGE
Activity
 The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/µg.Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.Activation Protocol:1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.3. Incubate at 37°C for 30 minutes.4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The MMP-3 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij35, pH 7.5.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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MMP3 HEK
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