MMLV RT

Alternative Names: Not Available

Recombinant Moloney Mouse Leukemia Virus Reverse Transcriptase (M-MuLV RT) is an enzyme widely used in molecular biology research for its ability to synthesize complementary DNA (cDNA) from RNA templates. Belonging to the retroviral family, M-MuLV RT is a key tool in reverse transcription, a process that converts RNA into DNA, enabling further analysis and manipulation. With a molecular weight of approximately 75 kDa, this enzyme plays a fundamental role in various applications, including gene expression studies, cloning, and PCR-based techniques. Its reliable performance and versatility make it a valuable asset in the field of molecular biology research.

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  • Sku: PSB-enz-310-2,500U
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Moloney Mouse Leukemia Virus Reverse Transcriptase (M-MuLV RT) is an enzyme widely used in molecular biology research for its ability to synthesize complementary DNA (cDNA) from RNA templates. Belonging to the retroviral family, M-MuLV RT is a key tool in reverse transcription, a process that converts RNA into DNA, enabling further analysis and manipulation. With a molecular weight of approximately 75 kDa, this enzyme plays a fundamental role in various applications, including gene expression studies, cloning, and PCR-based techniques. Its reliable performance and versatility make it a valuable asset in the field of molecular biology research.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System E. coli
Purity
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation 50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.

*For Research Use Only

Notes

5X Reaction Buffer: 250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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MMLV RT

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