mMDH2

Alternative Names: MDH, M-MDH, MOR1

MDH2 is an enzyme that plays a key role in the citric acid cycle by converting malate to oxaloacetate using the NAD/NADH cofactor system. It is found in the mitochondria and is involved in the malate-aspartate shuttle, which allows for communication between the cytosol and mitochondria. MDH2 is most abundant in the adrenal system, small intestine, heart and pancreas.

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  • Sku: PSB-enz-1068-2µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Mouse Malate Dehydrogenase 2 is an enzyme belonging to the malate dehydrogenase family. It plays a key role in the citric acid cycle by catalyzing the conversion of malate to oxaloacetate. This enzyme is essential for cellular respiration and energy production. Recombinant Mouse Malate Dehydrogenase 2 has a molecular weight of approximately 35 kDa. Its precise function and regulation make it a valuable target for research in metabolic pathways and mitochondrial dysfunction.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to Lmalate and beta-NAD per minute at pH8.0 at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 35.4 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation MDH2 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mMDH2
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