mLDHA

Alternative Names: Not Available

LDHA is an enzyme that plays a key role in the final step of anaerobic glycolysis by converting L-lactate and NAD to pyruvate and NADH. It is predominantly found in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been associated with exertional myoglobinuria. LDH1 levels are reduced in essential thrombocythemia. LDHA is triggered by estrogen through a non-genomic pathway. Lower LDH-A activity leads to increased mitochondrial respiration and decreased mitochondrial membrane potential.

  •
  • Sku: PSB-enz-952-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Mouse Lactate Dehydrogenase A is an enzyme belonging to the lactate dehydrogenase family, specifically the A subunit. This enzyme plays a critical role in the conversion of lactate to pyruvate during glycolysis. With a molecular weight of approximately 36 kDa, Recombinant Mouse Lactate Dehydrogenase A is commonly used in research and biotechnological applications for its ability to catalyze the interconversion of lactate and pyruvate.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System Sf9 - Baculovirus cells
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 37.5 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation LDHA protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

mLDHA
SUBHEADING

Recently viewed products