mIL12

Alternative Names: Edodekin-alpha, IL-12, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF.

IL-12 is a cytokine that promotes the production of IFNgamma from T-cells and natural killer cells. Additionally, it supports the differentiation of Th1 helper cells. It serves as a catalyst for cell-mediated immunity.

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  • Sku: PSB-cyt-144-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Mouse Interleukin-12 (IL-12) is a protein that belongs to the cytokine family. With a molecular weight of approximately 70 kDa, IL-12 plays a crucial role in the immune system by regulating the differentiation and function of T cells and natural killer cells. This recombinant form of IL-12 is produced through genetic engineering techniques, ensuring its purity and consistency for research and therapeutic applications. Its precise molecular structure and mechanism of action make it a valuable tool for studying immune responses and developing targeted therapies.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System HEK 293
Purity 95% by SDS-PAGE
Activity
IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Lyophilized
Formulation The protein was lyophilized from 0.5xPBS, pH 7.5.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.

Storage Instruction

Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized ;murine IL12 ;in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available

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mIL12

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