mHPGD

Alternative Names: 15-hydroxyprostaglandin dehydrogenase [NAD(+)] (EC:1.1.1.141), 15-PGDH, Hpgd, Pgdh1, Prostaglandin dehydrogenase 1.

HPGD is a crucial enzyme responsible for degrading prostaglandins. The 15-PGDH protein plays a key role in reducing the biological activity of prostaglandins by catalyzing the oxidation of the 15-hydroxyl group to a keto group. GDH1 is involved in various physiological and cellular processes, including inflammation.

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  • Sku: PSB-enz-1027-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Mouse Hydroxyprostaglandin Dehydrogenase 15-(NAD) is an enzyme belonging to the hydroxyprostaglandin dehydrogenase family. It plays a role in the metabolism of prostaglandins by catalyzing the oxidation of hydroxy groups in various prostaglandins. This enzyme has a molecular weight of approximately 35 kDa and is commonly used in research settings to study the regulation of prostaglandin levels in various biological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
31.6 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The HPGD solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMHVNGKV ALVTGAAQGI GKAFAEALLL HGAKVALVDW NLEAGVKCKA ALDEQFEPQK TLFVQCDVAD QKQLRDTFRK VVDHFGRLDI LVNNAGVNNE KNWEQTLQIN LVSVISGTYL GLDYMSKQNG GEGGIIINMS SLAGLMPVAQ QPVYCASKHG IIGFTRSAAM AANLMKSGVR LNVICPGFVD TPILESIEKE ENMGQYIEYK DQIKAMMKFY GVLHPSTIAN GLINLIEDDA LNGAIMKITA SKGIHFQDYD ISPLLVKAPL TS.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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mHPGD

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