mGSTP1

Alternative Names: Not Available

GSTP1 is a polymorphic gene known for encoding various GSTP1 variant proteins believed to play a role in xenobiotic metabolism and cancer susceptibility, among other diseases. Acting as a glutathione S-transferase belonging to the pi class, GSTP1 enzymes are involved in detoxification by conjugating hydrophobic and electrophilic compounds with reduced glutathione. These GST enzymes are categorized into 4 main classes (alpha, mu, pi, and theta) based on their properties. The reaction of glutathione with an acceptor molecule catalyzed by GSTP1 leads to the formation of a Sulfur-substituted glutathione, aiding in the transformation of various electrophiles. Inactivation of GSTP1 through CpG hypermethylation is common in pituitary adenomas and may contribute to aggressive tumor behavior. Additionally, GSTP1 is a potential transcriptional target of the p53 tumor suppressor gene and single-nucleotide polymorphisms in GSTP1 can affect protein binding, potentially impacting its role in carcinogen metabolism and disease pathogenesis. GST-pi may also play a key role in the proliferation of androgen-independent human prostate cancer cells.

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  • Sku: PSB-enz-909-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Mouse Glutathione S-Transferase pi 1 is a member of the glutathione S-transferase family, which plays a crucial role in detoxification processes within cells. With a molecular weight of approximately 23 kDa, this enzyme is involved in the conjugation of reduced glutathione to a wide variety of hydrophobic electrophiles. Recombinant Mouse Glutathione S-Transferase pi 1 is commonly used in research studies to investigate the mechanisms of drug metabolism and cellular protection against oxidative stress.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Specific activity is > 40 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GSTP1 protein solution (1mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPPYTIV YFPVRGRCEA MRMLLADQGQ SWKEEVVTID TWMQGLLKPT CLYGQLPKFE DGDLTLYQSN AILRHLGRSL GLYGKNQREA AQMDMVNDGV EDLRGKYVTL IYTNYENGKN DYVKALPGHL KPFETLLSQN QGGKAFIVGD QISFADYNLL DLLLIHQVLA PGCLDNFPLL SAYVARLSAR PKIKAFLSSP EHVNRPINGN GKQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mGSTP1
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