mGSTM1 His

Alternative Names: GST1, GTH4, GTM1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b, GSTM1, Glutathione S-transferase Mu 1, GST class-mu 1, Glutathione S-transferase GT8.7, pmGT10, GST 1-1.

There are two main families of glutathione transferase (GST) enzymes: cytosolic and membrane-bound. The soluble cytoplasmic mammalian GST enzymes fall into 8 classes: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. The mu class of GST enzymes play a key role in detoxifying electrophilic compounds, such as carcinogens, drugs, toxins, and oxidative stress products, by binding with glutathione. The genes responsible for the mu class enzymes are grouped together on chromosome 1p13.3 and exhibit significant genetic variation. These variations can impact an individual's susceptibility to toxins and carcinogens, as well as influence the effectiveness and potential side effects of certain drugs. Mutations in the mu class gene have been associated with an increased risk of developing certain cancers.

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  • Sku: PSB-enz-456-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Product Details

Product Description

Recombinant Mouse Glutathione S-Transferase M1 His-Tag is a protein belonging to the glutathione S-transferase family, which plays a key role in detoxification processes within cells. With a molecular weight of approximately 27 kDa, this protein is commonly used in research and biotechnological applications for its ability to bind to glutathione and facilitate the conjugation of various substrates. The His-Tag allows for easy purification and identification of the protein, making it a valuable tool for studying cellular processes related to detoxification mechanisms.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is < 11 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 28.1 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The GSTM1 solution contains 20 mM Tris-HCl buffer ( pH8.0), 1mM DTT and 10% glycerol

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mGSTM1 His
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