mGSTA1

Alternative Names: Not Available

Two supergene families encode membrane-bound and cytosolic forms of GST, which play a role in the detoxification of various compounds by conjugation with glutathione. Mammalian cytoplasmic GST enzymes fall into 8 classes: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. GSTA1, a member of the alpha class, is located on chromosome 6 and is mainly expressed in the liver, where it helps protect cells from reactive oxygen species.

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  • Sku: PSB-enz-873-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Mouse Glutathione S-Transferase Alpha 1 is a member of the glutathione S-transferase (GST) family, which plays a key role in detoxification processes by catalyzing the conjugation of reduced glutathione to a variety of electrophilic compounds. This protein has a molecular weight of approximately 26 kDa and is commonly used as a fusion tag in protein purification and detection assays due to its high affinity for glutathione. Recombinant Mouse GST Alpha 1 is widely utilized in research settings for its ability to facilitate the isolation and analysis of target proteins in a variety of biological samples.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 The specific activity is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C and is > 4,000 pmol/min/ug.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GSTA1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mGSTA1
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