mGOT1

Alternative Names: Not Available

GOT1 is an enzyme that relies on pyridoxal phosphate and can be found in cytoplasmic and mitochondrial forms as GOT1 and GOT2. These enzymes play a role in amino acid metabolism, urea cycle, and tricarboxylic acid cycle. Both enzymes are homodimeric and share a high degree of similarity.

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  • Sku: PSB-enz-872-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Mouse Glutamic-Oxaloacetic Transaminase 1, also known as GOT1, is an enzyme belonging to the aminotransferase family. This enzyme plays a critical role in amino acid metabolism by catalyzing the transfer of an amino group from aspartate to α-ketoglutarate to produce oxaloacetate and glutamate. With a molecular weight of approximately 45 kDa, Recombinant Mouse GOT1 is involved in various cellular processes, including energy production and nitrogen metabolism. Its recombinant form is commonly used in research studies to investigate the physiological functions and regulatory mechanisms of this enzyme in health and disease.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
48.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GOT1 protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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mGOT1

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