mGLO1

Alternative Names: Not Available

GLO1 is actively involved in the formation of S-lactoyl-glutathione through the catalysis of methylglyoxal condensation and reduced glutathione. It is localized on chromosome 6p21.3-p21.1 and is linked to the HLA gene. The enzyme GLO1 is highly expressed in various tumor cell lines, with its concentration often being increased ubiquitously. Moreover, GLO1 is identified as a major susceptibility gene for autism in an ethnic Chinese population. Additionally, GLO1 may play a role in the pathophysiology of mood disorders and is associated with kidney tumors through its overexpression.

  •
  • Sku: PSB-enz-953-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Mouse Glyoxalase-I is a protein belonging to the glyoxalase family, which plays a role in detoxifying methylglyoxal, a cytotoxic compound formed during glycolysis. This enzyme has a molecular weight of approximately 22 kDa and is involved in the conversion of methylglyoxal to D-lactate, utilizing glutathione as a cofactor. Recombinant Mouse Glyoxalase-I is commonly used in research studies to investigate the mechanisms of glyoxalase pathway and its implications in various diseases.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System Sf9 - Baculovirus cells
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 21.8 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GLO1 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

mGLO1
SUBHEADING

Recently viewed products