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mGAPDH, Active

Alternative Names: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, Gapdh, Gapd, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP.

GAPDH is a 36-kDa tetrameric enzyme that plays a key role in glycolysis by catalyzing the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate using NADPH. In addition to its glycolytic function in the cytoplasm, GAPDH is involved in various intracellular processes such as membrane fusion, microtubule bundling, and RNA export. It also catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and NAD, which is a critical energy-yielding step in carbohydrate metabolism.

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  • Sku: PSB-enz-986-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Product Details

Product Description

Recombinant Mouse Glyceraldehyde-3-Phosphate Dehydrogenase, Active is a member of the dehydrogenase enzyme family, specifically involved in glycolysis. This enzyme plays a crucial role in catalyzing the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. With a molecular weight of approximately 36 kDa, Recombinant Mouse Glyceraldehyde-3-Phosphate Dehydrogenase, Active is commonly used in biochemical research and drug development due to its high specificity and activity levels.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 40 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Tetramer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GAPDH protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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mGAPDH, Active
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