METAP1

Alternative Names: Methionine aminopeptidase 1, MetAP 1, Peptidase M 1, METAP1, KIAA0094, MAP1A, MetAP1A, MAP 1

METAP1, a member of the peptidase M24A family, functions by removing the amino-terminal methionine from nascent proteins. This enzyme preferentially releases N-terminal amino acids, specifically methionine, from peptides and arylamides. METAP1 is crucial for proper cell cycle progression due to its active site containing two adjacent divalent metal ions connected by a water molecule or hydroxide ion.

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  • Sku: PSB-enz-604-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Methionyl Aminopeptidase 1 is an enzyme belonging to the metalloprotease family. With a molecular weight of approximately 57 kDa, this enzyme plays a role in the regulation of protein synthesis by removing methionine residues from the N-terminus of newly synthesized proteins. Recombinant Human Methionyl Aminopeptidase 1 is commonly used in research settings to study protein degradation and post-translational modifications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
45.7 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The METAP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 3mM DTT, 40% glycerol, 200mM NaCl and 0.1mM PMSF.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMIKPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQDETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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METAP1

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