melA

Alternative Names: Alpha-galactosidase, JW4080, Mel-7, b4119

melA is a member of the glycosyl hydrolase 4 family and catalyzes the hydrolysis of saccharides with o-1,6,-galactoside bonds. While melA facilitates the same reaction in E. coli, human, and yeast, it is found in different cellular compartments: E. coli melA is cytoplasmic, while human and yeast melA are secretory proteins. Despite similarities in molecular weight and structure across the three species, differences are likely due to their divergent cellular locations.

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  • Sku: PSB-enz-609-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant E.Coli Alpha-Galactosidase is an enzyme derived from the bacterium Escherichia coli, belonging to the glycoside hydrolase family 27. This enzyme plays a crucial role in breaking down complex carbohydrates, specifically alpha-galactosides, into simpler sugars. With a molecular weight of approximately 45 kDa, Recombinant E.Coli Alpha-Galactosidase is commonly used in various industrial applications, such as food processing and pharmaceutical production, due to its ability to improve digestibility and reduce flatulence in individuals with conditions such as lactose intolerance.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
53.0 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The melA solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMMSAPKI TFIGAGSTIF VKNILGDVFH REALKTAHIA LMDIDPTRLE ESHIVVRKLM DSAGASGKIT CHTQQKEALE DADFVVVAFQ IGGYEPCTVT DFEVCKRHGL EQTIADTLGP GGIMRALRTI PHLWQICEDM TEVCPDATML NYVNPMAMNT WAMYARYPHI KQVGLCHSVQ GTAEELARDL NIDPATLRYR CAGINHMAFY LELERKTADG SYVNLYPELL AAYEAGQAPK PNIHGNTRCQ NIVRYEMFKK LGYFVTESSE HFAEYTPWFI KPGREDLIER YKVPLDEYPK RCVEQLANWH KELEEYKKAS RIDIKPSREY ASTIMNAIWT GEPSVIYGNV RNDGLIDNLP QGCCVEVACL VDANGIQPTK VGTLPSHLAA LMQTNINVQT LLTEAILTEN RDRVYHAAMM DPHTAAVLGI DEIYALVDDL IAAHGDWLPG WLHR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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melA

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