MDH

Alternative Names: EC 1.1.1.37, Malate dehydrogenase cytoplasmic, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375, MDH1.

Malate dehydrogenase is an enzyme that plays a crucial role in the citric acid cycle. It converts malate into oxaloacetate and can reverse this reaction as well. It is different from malic enzyme, which uses NADPH to convert pyruvate. Malate dehydrogenase is also involved in gluconeogenesis, which is the synthesis of glucose from smaller molecules. In this process, pyruvate is converted into oxaloacetate by pyruvate carboxylase in the mitochondria. To transport oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate. The malate then crosses the inner mitochondrial membrane and is oxidized back to oxaloacetate by cytosolic malate dehydrogenase in the cytosol. Finally, oxaloacetate is converted to phosphoenol pyruvate by phosphoenol-pyruvate carboxy kinase (PEPCK).

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  • Sku: PSB-enz-598-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Malate Dehydrogenase is an enzyme that plays a crucial role in the citric acid cycle, also known as the Krebs cycle, which is a fundamental metabolic pathway in all aerobic organisms. Belonging to the family of oxidoreductases, this enzyme catalyzes the reversible conversion of malate to oxaloacetate, coupled with the reduction of NAD+ to NADH. With a molecular weight of approximately 35-40 kDa, Recombinant Malate Dehydrogenase is of significant interest in various research fields, including biochemistry, molecular biology, and biotechnology.

Current Lead Time

7-14 Days

Product Specifications

Species
 Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 34.9 kDa
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The MDH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 50mM Nacl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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MDH
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