mDDT

Alternative Names: D-dopachrome tautomerase, D-dopachrome decarboxylase (EC:4.1.1.84), Ddt.

DDT is an enzyme that facilitates the conversion of D-dopachrome to 5,6-dihydroxyindole. It belongs to the family of lyases, specifically carboxy-lyases which break carbon-carbon bonds. DDT has a similar amino acid sequence to MIF and both have tautomerase activity. DDT also acts as a proinflammatory cytokine.

Skip to product information
  • Sku: PSB-enz-1073-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
 per 
Shipping calculated at checkout.

Contact us directly for international purchases.

Title

Product Details

Product Description

Recombinant Mouse D-Dopachrome Tautomerase, also known as DDT, is a member of the macrophage migration inhibitory factor (MIF) protein family. This protein plays a role in the regulation of immune responses and inflammation. With a molecular weight of approximately 12.5 kDa, Recombinant Mouse D-Dopachrome Tautomerase is involved in the catalysis of the conversion of D-dopachrome to 5,6-dihydroxyindole, a key step in the melanin biosynthesis pathway. Its function and structure make it a valuable target for research in immunology and dermatology.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
15.5 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation DDT protein solution (1mg/ml) contains 20mM Tris-Hcl buffer (pH8.0) & 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

View full details

mDDT

SUBHEADING

Recently viewed products