mCTSD

Alternative Names: CD, CatD, Cathepsin D, Ctsd

Cathepsin D is a lysosomal protease that plays a role in cancer cell migration and invasion by degrading the basement membrane, extracellular matrix, and connective tissue. It is synthesized as a 54kDa precursor, which undergoes proteolytic processing to form a 48kDa single chain intermediate, further maturing into stable 34kDa and 14kDa two chain forms. Its mitogenic and proteolytic activities make it a potential prognostic marker in various tumor types. Cathepsin D is expressed in both epithelial cells and macrophages, and its activity is regulated by estrogen.

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  • Sku: PSB-enz-1017-1µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Mouse Cathepsin-D is a member of the cathepsin family of proteases. This enzyme plays a critical role in intracellular protein degradation and processing. With a molecular weight of approximately 44 kDa, Recombinant Mouse Cathepsin-D is commonly used in research settings to study its enzymatic activity and potential therapeutic applications. Its ability to cleave peptide bonds in proteins makes it a valuable tool for investigating various biological processes and pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
Mouse
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
Specific activity is > 1,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
44.0 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CTSD protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

IIRIPLRKFT SIRRTMTEVG GSVEDLILKG PITKYSMQSS PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KILDIACWVH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC KSDQSKARGI KVEKQIFGEA TKQPGIVFVA AKFDGILGMG YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG GELMLGGTDS KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD KYILKVSQGG KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN RVGFANAVVL LEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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mCTSD

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