LACTB, His

Alternative Names: ampA, ampC, Beta-lactamase, Cephalosporinase, b4150, JW4111

Beta-lactamase (EC 3.5.2.6) is an enzyme found in certain bacteria that enables them to resist beta-lactam antibiotics. These antibiotics, including penicillins, cephalosporins, cephamycins, and carbapenems, all contain a common structural element known as a beta-lactam ring. The beta-lactamase enzyme is able to break this ring open, rendering the antibiotic ineffective in fighting bacterial infections.

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  • Sku: PSB-enz-088-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant E.Coli Beta Lactamase, His Tag is a protein belonging to the beta-lactamase family, specifically derived from Escherichia coli. This enzyme is commonly used in molecular biology research for its ability to hydrolyze beta-lactam antibiotics, rendering them ineffective. With a molecular weight of approximately 29 kDa, this protein is often tagged with a histidine (His) tag for purification purposes. Its high specificity and efficiency make it a valuable tool in various laboratory applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
41.8 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The LACTB enzyme (1mg/ml)is supplied in 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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LACTB, His

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