KRT14 His

Alternative Names: Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14, NFJ, CK14, EBS3, EBS4.

Cytokeratin 14 is part of the keratin family, which is the largest group of intermediate filaments. It is a type I keratin and is typically found in combination with two keratin 5 molecules, a type II keratin, to form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are linked to epidermolysis bullosa simplex. A pseudogene has been discovered at 17p12-p11.

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  • Sku: PSB-pro-941-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cytokeratin 14 His Tag is a protein product derived from genetic engineering techniques, specifically the insertion of a histidine (His) tag for purification purposes. Belonging to the cytokeratin family, it is a type I intermediate filament protein primarily expressed in epithelial cells. With a molecular weight of approximately 50 kDa, this protein plays a significant role in maintaining the structural integrity and mechanical stability of epithelial tissues. Its application in various research fields, including cell biology and dermatology, makes it a valuable tool for studying epithelial cell functions and related disorders.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Activity Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
53.8 kDa
Structure Heterotetramer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation KRT14 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIKDYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQNLEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN.

Storage Instruction

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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KRT14 His

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