KLK1, HEK

Alternative Names: KLK1, KLK-1, KLKR, KLK6, hKLK1, Tissue Kallikrein, Kidney/pancreas/salivary gland kallikrein, Kallikrein-1, HK1, HK-1, EC 3.4.21.35

Kallikreins are a group of serine protease enzymes with various physiological functions, including implications in carcinogenesis and potential as new cancer biomarkers. KLK1, located on chromosome 19, is one of fifteen kallikrein subfamily members. It releases Lys-bradykinin from low molecular weight kininogen, and is also known as Kallidinogenase, Kininogenase, or Kininogenin. KLK1, derived from humans or pigs, is used as a drug for mild to moderate hypertension and cerebral blood vessel occlusion. It exhibits selectivities for tyrosine/arginine, serine/arginine, and phenylalanine/leucine at specific sites. The rs5517 variant in the KLK1 gene is significantly associated with hypertension in the Chinese Han population. Endothelial cells express and release KLK1, which plays a role in maintaining circulation homeostasis. KLK1 also contributes to epidermal desquamation through the cleavage of desmoglein 1.

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  • Sku: PSB-enz-293-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Kallikrein-1, HEK is a member of the serine protease family, specifically kallikreins, which are enzymes involved in various physiological processes such as blood pressure regulation and inflammation. With a molecular weight of approximately 28 kDa, Recombinant Human Kallikrein-1, HEK is produced using human embryonic kidney cells (HEK) through recombinant DNA technology. This protein is commonly used in research and drug development for its role in the kallikrein-kinin system.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System HEK 293
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Provided as a solution in 20mM TrisHCl, 150mM NaCl, 2mM CaCl2, pH- 8.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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KLK1, HEK

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