IL12 His

Alternative Names: IL-12, Interleukin 12 (subunit beta/alpha), 23-219aa (IL12A) / 23-328aa (IL12B), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha.

IL-12 is a cytokine that facilitates interferon gamma production from T-cells and natural killer cells, while promoting the differentiation of Th1 helper cells. It is instrumental in initiating cell-mediated immunity.

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  • Sku: PSB-cyt-846-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Interleukin 12 His Tag is a genetically engineered protein used in research and therapeutic applications. Belonging to the cytokine family, it plays a significant role in regulating immune responses and inflammation. With a molecular weight of approximately 75 kDa, this protein is produced through recombinant DNA technology and contains a histidine (His) tag for purification purposes. Its availability and utilization in various studies make it a valuable tool for investigating immune system functions and developing potential immunotherapies.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Baculovirus
Purity 90% by SDS-PAGE
Activity
The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
23.3 kDa
Structure Heterodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation IL12 protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

IL12A(p35):RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH. IL12B(p40):IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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IL12 His

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