IDH1

Alternative Names: Isocitrate dehydrogenase [NADP] cytoplasmic, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, EC 1.1.1.42, IDH, NADP(+)-specific ICDH, IDP, PICD.

Isocitrate Dehydrogenase is an oxidoreductase enzyme that converts isocitrate and NAD+ to 2-ketoglutarate, carbon dioxide, and NADH. It is found in cell mitochondria and requires Mg2+ and Mn2+. The enzyme is activated by ADP, citrate, and Ca2+, while it is inhibited by NADH, NADPH, and ATP. This reaction is a crucial and rate-limiting step of the citric acid cycle.

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  • Sku: PSB-enz-289-1mg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Yeast Isocitrate Dehydrogenase-1 (IDH1) is an enzyme that plays a crucial role in the tricarboxylic acid (TCA) cycle, specifically catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate. Belonging to the family of isocitrate dehydrogenases, IDH1 is a key regulatory enzyme involved in cellular metabolism. With a molecular weight of approximately 45 kDa, this enzyme functions as a homodimer, utilizing NAD+ as a cofactor for its catalytic activity. Its significance lies in its contribution to the overall energy production and biosynthetic processes within cells.

Current Lead Time

7-14 Days

Product Specifications

Species
Yeast
Expression System Yeast cells
Purity 90 % by SDS-PAGE
Activity
The specific activity was found to be 115 U/mg. The specific activity was found to be 115 U/mg.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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IDH1

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