HSD17B8

Alternative Names: Arylsulfatase A, Cerebroside-Sulfatase, ASA, Metachromatic Leucodystrophy, MLD, EC 3.1.6.8

Arylsulfatase A is an enzyme that breaks down sulfatides, specifically the molecule cerebroside 3-sulfate, into cerebroside and sulfate. This enzyme is encoded by the ARSA gene in humans. The presence of phosphate can inhibit the enzyme by forming a covalent bond with the active site 3-oxoalanine.

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  • Sku: PSB-enz-108-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Hydroxysteroid (17-beta) Dehydrogenase 8 (HSD17B8) is an enzyme that plays a significant role in the metabolism of steroids and fatty acids. Belonging to the short-chain dehydrogenase/reductase (SDR) family, HSD17B8 is involved in the conversion of estradiol to estrone, a key step in estrogen biosynthesis. With a molecular weight of approximately 36 kDa, this enzyme facilitates the oxidation and reduction reactions required for the interconversion of hydroxysteroids. Its precise enzymatic activity and involvement in various physiological processes make HSD17B8 a subject of scientific interest and investigation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 95.0 % by SDS-PAGE
Activity
Specific activity is > 2,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
53.0 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ARSA protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADPRPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEVTVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPETMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQLDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HAHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HSD17B8

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