HS3ST1

Alternative Names: Heparan sulfate 3-O-sulfotransferase 1, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, h3-OST-1, HS3ST1, 3-OST-1, 3OST, 3OST1, HS3S1.

Heparan Sulfate 3-O-Sulfotransferase 1 (HS3ST1) is an enzyme that plays a key role in the biosynthesis of heparan sulfate (HS), an anticoagulant molecule. HS3ST1 utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to add a sulfo group to position 3 of glucosamine residues in heparan. This modification is essential for completing the structure of the antithrombin pentasaccharide binding site, a critical component in the anticoagulant function of heparan sulfate.

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  • Sku: PSB-enz-744-2µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1 is an enzyme belonging to the sulfotransferase family, specifically responsible for catalyzing the transfer of sulfate groups to heparan sulfate. This enzyme plays a crucial role in the modification of heparan sulfate, a complex polysaccharide involved in various biological processes. Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1 has a molecular weight of approximately 50 kDa, making it a key player in the sulfation of heparan sulfate chains.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 36.2 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation HS3ST1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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HS3ST1
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