HRP

Alternative Names: EC 1.11.1.7, Horseradish Peroxidase, HRP

Horseradish peroxidase (HRP), a potent enzyme found in horseradish, is extensively utilized in various molecular biology applications, including antibody amplification and detection. One prominent use of HRP is in marking neurons, a technique widely employed by neurobiologists. This method has gained significant popularity in recent years, surpassing even the Golgi stain, which has been in use since its discovery in 1870. Moreover, HRP is widely exploited in techniques such as Western blotting and ELISAs. In immunoassays, HRP serves as a widely employed enzymatic label. Typically, it is conjugated to antibodies, lectins, or haptens, with coupling achieved through the carbohydrate side chains of HRP.

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  • Sku: PSB-enz-321-10mg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Horseradish Peroxidase (HRP) is an enzyme derived from the roots of the horseradish plant (Armoracia rusticana). Belonging to the heme peroxidase family, HRP is a glycoprotein with a molecular weight of approximately 44 kDa. This enzyme plays a significant role in various biochemical assays and immunohistochemical staining techniques due to its ability to catalyze the oxidation of a wide range of substrates. Its robust enzymatic activity and stability make HRP a valuable tool in scientific research, diagnostics, and biotechnology applications.

Current Lead Time

7-14 Days

Product Specifications

Species
enzyme
Expression System Root extracts of horseradish
Purity
Activity
276 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Lyophilized
Formulation Contact for Formulation

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 µg/ml.

References

Not Available

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HRP

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