HPSE Active

Alternative Names: Not Available

Heparanase is an enzyme that breaks down heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. This process is important for the degradation of the extracellular matrix, facilitating the movement of tumor cells and inflammatory leukocytes. As heparanase degrades heparan sulfate, it releases growth factors and cytokines that promote cell proliferation and chemotaxis. Heparanase is composed of two subunits, a 50 kDa subunit with the active site and an 8 kDa subunit. It is initially produced as a 65 kDa precursor and undergoes proteolytic processing to become active. Heparanase is highly expressed in myeloid leukocytes, platelets, and human placenta. In various types of primary tumors, human heparanase is upregulated and is associated with increased invasiveness, vascularity, and poor survival rate.

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  • Sku: PSB-enz-1032-1µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human Heparanase-1 Active is a biotechnological product that belongs to the family of enzymes known as heparanases. With a weight of approximately X kDa, this enzyme plays a significant role in the degradation of heparan sulfate, a polysaccharide found in the extracellular matrix. By cleaving heparan sulfate chains, Recombinant Human Heparanase-1 Active facilitates various physiological and pathological processes, including cell migration, angiogenesis, and tumor metastasis. Its precise mechanism of action and potential therapeutic applications make this enzyme a subject of scientific interest and investigation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Contact for Details
Purity 95.0 % by SDS-PAGE
Activity
 The specific activity of Heparanase Active Enzyme in-house standard is about 0.7 Units (1 unit = 1 μmole of reducing ends of heparan sulfate substrate formed per minute per mg Heparanase Active Enzyme at 37°C). The enzymatic activity of each Heparanase Active Enzyme batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically. Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation Heparanase Active Enzyme is supplied in20mM Acetate buffer and 750mM NaCl pH 5.4.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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HPSE Active
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