HNMT, Active

Alternative Names: HMT, HNMT-S1, HNMT-S2, MRT51

Histamine N-Methyltransferase (HNMT) is an enzyme found in the cell cytosol that uses S-adenosyl-L-methionine as a methyl donor to inactivate histamine. This inactivation of histamine impacts the immune system's response. HNMT is active in various body tissues like the kidney, central nervous system, and bronchus. Its role is critical in regulating the airway response to histamine and the degradation process of histamine.

  •
  • Sku: PSB-enz-1071-1µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Histamine N-Methyltransferase is an enzyme belonging to the methyltransferase family, specifically involved in the methylation of histamine. This enzyme plays a key role in the regulation of histamine levels in the body. With a molecular weight of approximately 34 kDa, Recombinant Human Histamine N-Methyltransferase is crucial for the metabolism of histamine and its subsequent inactivation. Its recombinant form is commonly used in research and pharmaceutical applications for studying histamine-related pathways and developing potential therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90.0 % by SDS-PAGE
Activity
 Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 37.4 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation HNMT protein solution (1mg/ml) containing 20mM, Tris-Hcl (pH8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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HNMT, Active
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