HNMT

Alternative Names: Histamine N-methyltransferase, HNMT, HNMT-S1, HNMT-S2, HMT

HNMT, located in the cytosol, utilizes S-adenosyl-L-methionine as a methyl donor. In the mammalian brain, N(tau)-methylation plays a crucial role in controlling histamine neurotransmitter activity due to the absence of diamine oxidase in the central nervous system. A genetic polymorphism affects the activity levels of the HNMT gene product in red blood cells. HNMT functions to deactivate histamine through n-methylation, thereby regulating the body's response to histamine. Histamine plays a role in regulating the immune response by stimulating four types of receptors (H1, H2, H3, and H4) present on target cells. HNMT also deactivates histamine in the bronchus, kidney, and central nervous system.

  •
  • Sku: PSB-enz-402-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Histamine N-Methyltransferase is an enzyme that belongs to the methyltransferase family. With a molecular weight of approximately 33 kDa, this enzyme plays a crucial role in the metabolism of histamine by catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to histamine, leading to the formation of N-methylhistamine. This process is essential for regulating histamine levels in the body and maintaining homeostasis.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The HNMT solution contains 20mM Tris-HCl pH-8 and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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HNMT
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