HIBCH

Alternative Names: EC 3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase mitochondrial, HIB-CoA hydrolase, HIBYL-CoA-H

The HIBCH enzyme plays a crucial role in the hydrolysis of HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in the HIBCH gene are associated with 3-hydroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in the presence of multiple transcript variants.

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  • Sku: PSB-enz-594-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human 3-Hydroxyisobutyryl-CoA Hydrolase is an enzyme that belongs to the hydrolase family. This enzyme plays a key role in the catabolism of valine and isoleucine by catalyzing the hydrolysis of 3-hydroxyisobutyryl-CoA into 3-hydroxyisobutyrate and CoA. With a molecular weight of approximately 35 kDa, Recombinant Human 3-Hydroxyisobutyryl-CoA Hydrolase is involved in the regulation of metabolic pathways related to branched-chain amino acids.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
42.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The HIBCH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMDAAEE VLLEKKGCTG VITLNRPKFL NALTLNMIRQ IYPQLKKWEQ DPETFLIIIK GAGGKAFCAG GDIRVISEAE KAKQKIAPVF FREEYMLNNA VGSCQKPYVA LIHGITMGGG VGLSVHGQFR VATEKCLFAM PETAIGLFPD VGGGYFLPRL QGKLGYFLAL TGFRLKGRDV YRAGIATHFV DSEKLAMLEE DLLALKSPSK ENIASVLENY HTESKIDRDK SFILEEHMDK INSCFSANTV EEIIENLQQD GSSFALEQLK VINKMSPTSL KITLRQLMEG SSKTLQEVLT MEYRLSQACM RGHDFHEGVR AVLIDKDQSP KWKPADLKEV TEEDLNNHFK SLGSSDLKF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HIBCH

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