HARS Sf9

Alternative Names: Histidyl-tRNA synthetase, Histidine-tRNA ligase, EC 6.1.1.21, HisRS, HRS, FLJ20491, JO-1.

Aminoacyl-tRNA synthetases are enzymes that attach amino acids to tRNAs. This gene encodes a cytoplasmic enzyme that is part of the class II family of aminoacyl-tRNA synthetases. It specifically helps in the synthesis of histidyl-transfer RNA, which is crucial for adding histidine to proteins. The gene is located on chromosome five opposite HARSL, sharing a promoter. This gene is often targeted by autoantibodies in the autoimmune disease polymyositis/dermatomyositis.

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  • Sku: PSB-enz-335-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Histidyl-tRNA Synthetase Sf9 is a member of the aminoacyl-tRNA synthetase family, responsible for catalyzing the attachment of histidine to its cognate tRNA molecule in protein synthesis. This enzyme has a molecular weight of approximately 60 kDa and is commonly used in research and biotechnological applications for its role in accurately translating genetic information into functional proteins. Recombinant Human Histidyl-tRNA Synthetase Sf9 offers a reliable and efficient tool for studying the mechanisms of translation and protein synthesis in various biological systems.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Insect cells
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HARS Sf9

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