HARS

Alternative Names: Histidyl-tRNA synthetase, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1, HARS, EC 6.1.1.21

Aminoacyl-tRNA synthetases are enzymes that attach amino acids to tRNAs. The specific enzyme discussed here belongs to the class II family and is responsible for attaching histidine to tRNAs. This gene is located on chromosome five alongside another gene with a shared promoter. Mutations in this gene are associated with autoimmune diseases.

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  • Sku: PSB-enz-268-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Histidyl-tRNA Synthetase is an enzyme responsible for catalyzing the attachment of histidine to its cognate transfer RNA molecule during protein synthesis. Belonging to the aminoacyl-tRNA synthetase family, this enzyme plays a key role in the accurate translation of genetic information from mRNA to protein. With a molecular weight of approximately 55 kDa, Recombinant Human Histidyl-tRNA Synthetase is crucial for maintaining cellular homeostasis and proper functioning of the translational machinery.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The protein solution contains 500mM NaCl and 10mM Tris (pH 8.0) and 6M Urea.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HARS

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