HAO1, Active

Alternative Names: Hydroxyacid oxidase 1, HAO1, Glycolate oxidase, GOX, HAOX1, GOX1.

Glycolate oxidase (HAO1) is an alpha hydroxy acid oxidase enzyme that catalyzes the oxidation of glycolate to glyoxylate and glyoxylate to oxalate using flavin mononucleotide (FMN) as a mediator. HAO1 is primarily found in the liver and pancreas and shows high activity with two-carbon substrates like glycolate. HAO1 is linked to hyperoxaluria, a condition characterized by the accumulation of calcium oxalate kidney stones.

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  • Sku: PSB-enz-1094-2µg
  • Vendor: ProSpec-Tany
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UniProt Listing
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Product Details

Product Description

Recombinant Human Hydroxyacid Oxidase 1, Active is an enzyme belonging to the hydroxyacid oxidase family. With a molecular weight of approximately 32 kDa, this enzyme plays a key role in catalyzing the oxidation of hydroxyacids. Its active form is commonly used in research and biotechnological applications for studying metabolic pathways and drug development.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 3000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 45.0 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation HAO1 protein solution (1mg/ml) contains 20% glycerol, 20mM Tris-Hcl (pH8.0) and 0.5M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRMLRNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLVRQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIVAKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQGEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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HAO1, Active
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