HAO1

Alternative Names: Hydroxyacid oxidase 1, HAO1, Glycolate oxidase, GOX, HAOX1, GOX1.

Glycolate oxidase (HAO1) is an enzyme in the alpha hydroxy acid oxidases (HAO) superfamily. HAO1 catalyzes the oxidation of glycolate to glyoxylate and glyoxylate to oxalate using FMN, while also reducing oxygen to hydrogen peroxide. It is predominantly found in the liver and pancreas, showing high activity on two-carbon substrates like glycolate. Recent research has linked HAO1 to hyperoxaluria, a condition characterized by the formation of kidney stones due to increased calcium oxalate deposits.

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  • Sku: PSB-enz-162-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Hydroxyacid Oxidase 1 is an enzyme that belongs to the family of oxidoreductases. This enzyme plays a key role in the metabolism of hydroxyacids by catalyzing the oxidation of L-lactate and glycolate to pyruvate and glyoxylate, respectively. Recombinant Human Hydroxyacid Oxidase 1 has a molecular weight of approximately 32 kDa, making it a relatively small enzyme with significant metabolic implications. Its enzymatic activity is essential for the proper functioning of various metabolic pathways in the human body.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation HAO1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.5M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HAO1

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