HAGH

Alternative Names: Glyoxalase II, GLX2, GLXII, GLO2, Hydroxyacyl Glutathione Hydrolase, HAGH1, hydroxyacylglutathione hydroxylase, Hydroxyacylglutathione Hydrolase.

HAGH is a member of the glyoxalase enzyme family that acts as a thiolesterase, breaking down S-lactoyl-glutathione into reduced glutathione and D-lactate. It is important in detoxifying the byproduct methylglyoxal from glycolysis and is regulated by p63 and p73 as a pro-survival factor of the p53 family. HAGH functions as a monomer and binds two zinc ions per subunit.

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  • Sku: PSB-enz-034-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Hydroxyacylglutathione Hydrolase is an enzyme that belongs to the thioesterase enzyme family. It plays a role in the breakdown of lipid peroxides and detoxification of reactive aldehydes in the cell. With a molecular weight of approximately 39 kDa, this enzyme is involved in the maintenance of cellular redox balance and protection against oxidative stress. Its recombinant form is commonly used in research and biotechnological applications for its ability to catalyze the hydrolysis of hydroxyacylglutathione substrates.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
31.4 kDa
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The HAGH protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.5) and 10% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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HAGH

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