GZMB, Sf9

Alternative Names: Granzyme B, Granzyme 2, Granzyme-2, EC 3.4.21, CCPI, CSP-B, CGL-1, CTLA-1, C11, SECT, CGL1, CSPB, CTLA1, CTSGL1, Fragmentin-2, Lymphocyte Protease, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, HLP, Cytotoxic Serine Protease B, Cytotoxic T-Lymphocyte Proteinase 2, Human Lymphocyte Protein, EC 3.4.21.79, Fragmentin 2, Cathepsin G-Like 1, T-Cell Serine Protease 1-3E, GRB.

Granzyme-B (GZMB) plays a crucial role in cell-mediated immune responses by inducing target cell lysis through activating caspases responsible for apoptosis execution. GZMB cleaves caspase-3, -7, -9, and 10 to trigger enzymes that promote apoptosis.

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  • Sku: PSB-enz-1078-1µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Granzyme-B, Sf9 is a member of the granzyme family, which plays a key role in immune response by inducing apoptosis in target cells. This specific protein is expressed in Spodoptera frugiperda (Sf9) insect cells and has a molecular weight of approximately 29 kDa. Recombinant Human Granzyme-B, Sf9 is commonly used in research and drug development for its ability to induce cell death in a specific and controlled manner.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Baculovirus cells
Purity 95.0 % by SDS-PAGE
Activity
 Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 26.5 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GZMB protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) ,20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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GZMB, Sf9
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