GST S. Japonicum

Alternative Names: Not Available

Glutathione S-transferase (GST) is an antioxidant enzyme that plays a key role in defending cells against reactive oxygen species. It is responsible for reducing lipid hydroperoxides through glutathione peroxidase activity, independent of selenium. GST also helps detoxify lipid peroxidation byproducts like 4-hydroxynonenal.

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  • Sku: PSB-enz-1147-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Schistosoma japonicum glutathione S-transferase is a protein belonging to the glutathione S-transferase family. With a molecular weight of approximately 26 kDa, this enzyme plays a role in detoxification processes by catalyzing the conjugation of reduced glutathione to a wide variety of hydrophobic electrophiles. Its recombinant form is commonly used in research and biotechnology applications due to its ability to efficiently bind and modify various substrates.

Current Lead Time

7-14 Days

Product Specifications

Species
Schistosoma
Expression System E. coli
Purity 90.0 by SDS-PAGE
Activity
Specific activity is > 30unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GST S. Japonicum protein solution (1mg/ml) contains Phosphate-Buffered Saline (pH 7.4)and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GST S. Japonicum

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