GST His

Alternative Names: Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 28 kDa isozyme, GST 28, EC 2.5.1.18, Sj28GST, Sj28 antigen.

Glutathione S-Transferase (GST) is an antioxidant enzyme that is essential for cellular defense against reactive oxygen species. GST has glutathione peroxidase activity which helps reduce lipid hydroperoxides and detoxify lipid peroxidation end products like 4-hydroxynonenal (4-HNE). GST is a 26 kDa dimeric protein found in all aerobic organisms. It consists of two domains: one that binds GSH and is similar in structure to thioredoxin, and another that binds hydrophobic substrates. The GST-fusion protein expression system is commonly used to express peptides or protein domains fused to the C-terminus of Schistosoma japonicum GST. Fusion proteins retain GST enzymatic activity and can be purified using GST-affinity column chromatography. After purification, the desired peptides or domains can be separated from GST using a specific protease. This technique is widely used for various applications such as protein crystallization, molecular immunology studies, vaccine production, and protein-protein or protein-DNA interaction studies.

  •
  • Sku: PSB-enz-451-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant E.Coli Glutathione S-Transferase His Tag is a protein commonly used in molecular biology research for protein purification and detection. Belonging to the glutathione S-transferase family, this protein has a molecular weight of approximately 26 kDa. Its fusion with a histidine tag allows for easy purification using immobilized metal affinity chromatography. This versatile protein is widely utilized in various biochemical assays and protein-protein interaction studies due to its high affinity for glutathione and efficient purification properties.

Current Lead Time

7-14 Days

Product Specifications

Species
 Bacteria
Expression System E. coli
Purity 90 % by SDS-PAGE
Activity
 >10 units/mg, & is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH-6.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 28.3 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GST is supplied in PBS pH 7.4 & 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

GST His
SUBHEADING

Recently viewed products