GST

Alternative Names: Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST, Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST.

Glutathione S-Transferase (GST) is an essential antioxidant enzyme that plays a crucial role in cellular defense against reactive oxygen species. GST reduces lipid hydroperoxides and detoxifies lipid peroxidation end products. It is a 26 kDa dimeric protein found in all aerobic organisms, with each monomer consisting of two domains - one binding GSH and the other binding the hydrophobic substrate. The GST fusion protein expression system is commonly used for recombinant protein expression, allowing peptides or regulatory protein domains to be expressed as a fusion with GST. Fusion proteins retain GST enzymatic activity and can be purified via affinity column chromatography. The technique is widely utilized for various applications in biotechnology and research, such as protein crystallization, immunology studies, vaccine production, and studying protein interactions.

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  • Sku: PSB-enz-393-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant E.Coli Glutathione S-Transferase is a protein commonly used in molecular biology research for its ability to catalyze the conjugation of reduced glutathione to a wide variety of electrophilic compounds. Belonging to the glutathione S-transferase family, this enzyme plays a key role in detoxification processes within cells. With a molecular weight of approximately 26 kDa, Recombinant E.Coli Glutathione S-Transferase is a valuable tool for studying protein-protein interactions and drug metabolism pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
 Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is >20 units/mg. A unit is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C. Specific activity is >20 units/mg. A unit is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GST supplied in Phosphate Buffered Saline pH 7.4.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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GST
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