GSR

Alternative Names: Glutathione reductase mitochondrial, GR, GSR, GLUR, GRase, GRD1

Glutathione reductase (GSR) is an enzyme that belongs to a family of pyridine nucleotide-disulfide oxidoreductases. This homodimeric flavoprotein plays a crucial role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG). The main function of GSR in most eukaryotic cells is to regulate the ratio of [GSH] / [GSSG] and it is involved in activities such as detoxification of reactive oxygen species and biosynthesis of proteins and DNA.

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  • Sku: PSB-enz-202-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Glutathione Reductase is an enzyme that belongs to the oxidoreductase family. It plays a critical role in maintaining cellular redox homeostasis by catalyzing the reduction of glutathione disulfide to its active form, glutathione. This enzyme has a molecular weight of approximately 54 kDa and is essential for protecting cells from oxidative stress. Recombinant Human Glutathione Reductase is commonly used in research and biotechnology applications for studying redox signaling pathways and developing antioxidant therapies.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Specific activity: > 29 unit/ml. One unit will reduce 1.0 umol of oxidized glutathione per minute at pH 7.5 at 25°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
54.3 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The GSR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GSR

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