GPT, Active

Alternative Names: Alanine aminotransferase 1, ALT1, AAT1, EC 2.6.1.2, Glutamate pyruvate transaminase 1, GPT 1, GPT, GPT1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1.

Alanine transaminase, also known as ALT, is an enzyme found in serum and various bodily tissues, with the highest concentration in the liver. ALT catalyzes the transfer of an amino group from alanine to a-ketoglutarate, producing pyruvate and glutamate. Clinically, ALT is measured as part of a liver function test to assess liver health. It is also referred to as serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT) and is typically measured in units per liter (U/L).

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  • Sku: PSB-enz-280-1U
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Glutamic-Pyruvate Transaminase, Active is an enzyme belonging to the transaminase family, which plays a key role in amino acid metabolism. This enzyme has a molecular weight of approximately 45 kDa and is involved in the transfer of amino groups between glutamate and pyruvate. Recombinant Human Glutamic-Pyruvate Transaminase, Active is commonly used in biochemical research and diagnostic assays to study liver function and detect liver damage.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
The specific activity was found to be 839 U/mg.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GPT, Active

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