GPD2

Alternative Names: Glycerol-3-phosphate dehydrogenase mitochondrial, glycerol-3-phosphate dehydrogenase 2 (mitochondrial), GPDH-M, GPD-M, mtGPD, GPD2, GDH2, GPDM, mGPDH.

GPD2 is a Ca2+-sensitive, FAD-binding protein located on the outer surface of the inner mitochondrial membrane. It has two isoforms, with isoform 1 containing 727 a.a. residues and isoform 2 lacking 126 a.a. residues of the N-terminus. GPD2 catalyzes the oxidation of glycerol-3-phosphate to DHAP while reducing the enzyme-bound FAD. It acts as a testis-specific promoter of mitochondrial GPDH and participates in the glycerol phosphate shuttle, transferring reducing equivalents into mitochondria. GPD2 deficiency impairs glucose-stimulated insulin secretion and its up-regulation in highly glycolytic environments may increase ROS generation and contribute to the progression of prostate cancer.

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  • Sku: PSB-enz-437-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2 is an enzyme that plays a key role in glycerol metabolism. Belonging to the dehydrogenase family, this protein catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, a critical step in both glycolysis and gluconeogenesis pathways. With a molecular weight of approximately 37 kDa, Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2 is essential for cellular energy production and lipid biosynthesis.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
66.26 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GPD2 protein solution is supplied in 20mM Tris-HCl pH 8, 1mM EDTA and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GPD2

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