GNMT Active

Alternative Names: Glycine N-methyltransferase, GNMT, Glycine N-Methyltransferase, EC 2.1.1.20, Epididymis Secretory Sperm Binding Protein Li 182mP, HEL-S-182mP.

GNMT is a cytoplasmic enzyme that plays a crucial role in catalyzing the conversion of S-adenosyl-L-methionine with glycine to S-adenosyl-L-homocysteine and sarcosine. Defects in the GNMT gene can lead to GNMT deficiency (hyper methioninemia). GNMT is involved in regulating DNA methylation by controlling the balance between S-adenosylmethionine and S-adenosylhomocysteine. It also participates in the detoxification process in liver cells. Decreased expression of GNMT has been observed in human hepatocellular carcinoma. GNMT is responsible for the methylation of glycine using S-adenosylmethionine to produce N-methylglycine (sarcosine) and S-adenosylhomocysteine. Overall, GNMT plays a critical role in maintaining tissue concentration of adomet and regulating methionine metabolism.

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  • Sku: PSB-enz-1059-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Product Details

Product Description

Recombinant Human Glycine N-Methyltransferase, Active is a member of the methyltransferase enzyme family. This enzyme plays a role in the methylation of glycine to form sarcosine, a key step in the one-carbon metabolism pathway. With a molecular weight of approximately 35 kDa, Recombinant Human Glycine N-Methyltransferase, Active is commonly used in biochemical research and drug development studies. Its enzymatic activity makes it a valuable tool for investigating metabolic pathways and potential therapeutic targets.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
 Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 34.9 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation GNMT protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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GNMT Active
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