GLUD1

Alternative Names: Glutamate Dehydrogenase 1, Glutamate Dehydrogenase 1 Mitochondrial, Glutamate Dehydrogenase (NAD(P)+), GDH, GDH1, GDH 1, GLUD, GLUD1, EC 1.4.1, EC 1.4.1.3

GLUD1, an enzyme within the Glu/Leu/Phe/Val dehydrogenases family, is responsible for converting L-glutamate into alpha-ketoglutarate. It plays a crucial role in nitrogen metabolism across all organisms by catalyzing the oxidative deamination of 1-glutamate to 2-oxoglutarate. Additionally, GLUD1 is involved in regulating amino acid induced insulin secretion. Mutations in the GLUD1 gene lead to hyperinsulinism-hyperammonemia syndrome (HHS), characterized by elevated insulin and ammonia levels. The enzyme is allosterically affected by ADP, GTP, and ATP.

  •
  • Sku: PSB-enz-792-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Glutamate Dehydrogenase 1 is an enzyme belonging to the glutamate dehydrogenase family. It plays a key role in the metabolism of glutamate by catalyzing the reversible conversion of glutamate to alpha-ketoglutarate and ammonia. This enzyme has a molecular weight of approximately 48 kDa and is involved in the regulation of cellular energy production and nitrogen metabolism. Recombinant Human Glutamate Dehydrogenase 1 is commonly used in research and biotechnology applications for studying glutamate metabolism and related pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 80.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 58.4 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The GLUD1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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GLUD1
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