GLRX5

Alternative Names: GLRX5, glutaredoxin 5, GRX5, Monothiol glutaredoxin-5, MGC14129, FLB4739, C14orf87, PR01238, PRO1238, Glutaredoxin-related protein 5 mitochondrial

GLRX5 is a small redox enzyme found in mitochondria that is approximately 100 amino acids in size. It utilizes glutathione as a cofactor and plays a crucial role in the biogenesis of iron-sulfur clusters necessary for normal iron homeostasis. This enzyme is essential for regulating hemoglobin synthesis through interactions with the iron-sulfur protein ACO1. Mutations in the GLRX5 gene can lead to anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA).

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  • Sku: PSB-enz-480-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Glutaredoxin 5 is a member of the glutaredoxin family, a group of small redox enzymes involved in regulating cellular thiol-disulfide balance. With a molecular weight of approximately 15 kDa, Glutaredoxin 5 plays a key role in maintaining redox homeostasis within the cell by catalyzing the reduction of disulfide bonds in target proteins. This recombinant protein is commonly used in research settings to study the mechanisms of oxidative stress and redox signaling pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation GLRX5 solution containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GLRX5

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