glpE

Alternative Names: Thiosulfate sulfurtransferase GlpE, ECK3411, JW3388, b3425

The enzyme thiosulfate sulfurtransferase (glpE) is found in the mitochondrion and is produced by the nucleus. In Escherichia coli, glpE belongs to the rhodanese superfamily and facilitates the transfer of sulfur from thiosulfate to cyanide through a double-displacement mechanism.

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  • Sku: PSB-enz-714-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant E. coli Thiosulfate sulfurtransferase is an enzyme that belongs to the rhodanese family. It plays a role in sulfur metabolism by transferring sulfur atoms between molecules. With a molecular weight of approximately 33 kDa, this enzyme is involved in various cellular processes related to sulfur-containing compounds. Its recombinant form allows for easier study and manipulation in research settings.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
14.5 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The glpE solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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glpE

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