GLO1

Alternative Names: GLO1, Glyoxalase-1, Glyoxalase I, Glx I, GLYI, GLOD1, Methylglyoxalase, Aldoketomutase, Ketone-aldehyde mutase, Lactoylglutathione lyase, S-D-lactoylglutathione methylglyoxal lyase

GLO1 is an enzyme involved in the formation of S-lactoyl-glutathione from methylglyoxal and reduced glutathione. It is located on chromosome 6p21.3-p21.1 and is associated with HLA. GLO1 is highly expressed in tumor cell lines, and its levels are often increased ubiquitously. In Taiwanese individuals of Chinese descent, GLO1 is a significant genetic factor for autism. The enzyme may also play a role in mood disorders and kidney tumors.

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  • Sku: PSB-enz-398-10µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Glyoxalase-I is an enzyme that plays a key role in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Belonging to the glyoxalase family, this enzyme is crucial for maintaining cellular homeostasis by preventing the accumulation of harmful reactive carbonyl compounds. With a molecular weight of approximately 22 kDa, Recombinant Human Glyoxalase-I is a promising target for therapeutic interventions aimed at mitigating oxidative stress and diabetic complications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Glyoxalase-1 solution containing 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIAWALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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GLO1

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